Analysis Of Phytochemical Profile And Antioxidant Activities From Ethanol Extract Of Leaf, Stem Bark And Wood Clausena lansium L.
Analisis Profil Fitokimia Dan Aktivitas Antioksidan Ekstrak Etanol Daun, Kayu Dan Kulit Batang Tumbuhan Clausena lansium L.
Abstract
Wampi belongs to Rutaceae family. The shape of this plant is kind of bush or small tree and its fruits are similar to grapples or oranges. C. lansium was reported has the main biological activity as radical scavenger. The aim of this study to identify the content of secondary metabolites found in bark, woody stems and leaves of C. lansium plants, and to determine antioxidant activity through IC50 values. This research method begins with sample preparation, plant determination, simplicia characteristic examination, extraction, characterization with infrared spectroscopy, thin layer chromatography (TLC), then proceed with antioxidant activity assay using DPPH method (2.2 -Diphenyl-1-picrylhidrazyl) with a comparison of vitamin C. The results of this study showed that C. lansium contains alkaloids and kumarin. Furthermore, antioxidant activity assay measured absorption at a wavelength of 517 nm, obtained IC50 values of vitamin C 6.22 ppm; bark 231.54 ppm (weak), wood stem 131.49 ppm (weak), and leaves 22,60 ppm (strong).
Keywords: Clausena lansium, Antioxidant, phytochemical profile.
References
Ismail AA, Ahmad BA, Mohamed A, RasedeeAbdullah, Siddig IA, Mohamed YI, et al. A review of traditional uses, phytochemical and pharmacological aspects of selected members of Clausena genus (Rutaceae). J Med Plants Res. 2012. 6(38):5107–18. http://dx.doi.org/10.5897/jmpr12.317
Prasad KN, Hao J, Yi C, Zhang D, Qiu S, Jiang Y, et al. Antioxidant and anticancer activities of wampee (Clausena lansium (Lour.) Skeels) peel. J Biomed Biotechnol. 2009/07/30. 2009. 2009:612805. https://pubmed.ncbi.nlm.nih.gov/19657451
Maneerat W, Prawat U, Saewan N, Laphookhieo S. New coumarins from Clausena lansium twigs. J Braz Chem Soc. 2010.21(4):665–8. http://dx.doi.org/10.1590/s0103-50532010000400012
Liu J, Li C-J, Du Y-Q, Li L, Sun H, Chen N-H, et al. Bioactive Compounds from the Stems of Clausena lansium. Molecules. 2017. 22(12):2226. https://pubmed.ncbi.nlm.nih.gov/29240703
Adebajo AC, Iwalewa EO, Obuotor EM, Ibikunle GF, Omisore NO, Adewunmi CO, et al. Pharmacological properties of the extract and some isolated compounds of Clausena lansium stem bark: Anti-trichomonal, antidiabetic, anti-inflammatory, hepatoprotective and antioxidant effects. J Ethnopharmacol. 2009. 122(1):10–9. http://dx.doi.org/10.1016/j.jep.2008.11.015
