Antibacterial Activity of the Essential Oil from Betel leaf (Piper betle L.) against Streptococcus pyogenes and Staphylococcus aureus

UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN SIRIH HIJAU (Piper betle L.) TERHADAP BAKTERI Streptococcus pyogenes dan Staphylococcus aureus

Abstract

Betel leaf (Piper betle L) is a medicinal plant. Its essential oil has antibacterial activity of phenolic compounds and theirs derivatives that can inhibit a wide range of bacteria. Streptococcus pyogenes and Staphylococcus aureus is a bacteria that can cause strep throat (pharyngitis). This study was conducted to determine how to obtain the essential oil of Betel leaf green (Piper betle L.) using steam-water distillation method and determine the antibacterial activity of essential oils of betel leaf (Piper betle L.) against the bacteria Streptococcus pyogenes and Staphylococcus aureus using wells method. Betel leaf 5 kg isolated using steam-water distillation of essential oils produces as much as 9 mL, yield of 0.135%, a specific gravity of 0.750 g / mL, and the refractive index of 1.337. The results of GC-MS analysis of betel leaf green essential oil obtained 10 Most compounds are compounds chavicol, eugenol, germacrene D, caryophyllen, eugenol acetate, 2-allyphenol, β-chamigrene, α-cadinene, terpineol, and α-humulene. Based on the test results of the antibacterial activity of betel leaf that the essential oil of betel leaf green (Piper betle L) at the concentration of 10%, 20%, 30%, and 40% can inhibit the bacteria Streptococcus pyogenes and Staphylococcus aureus, the category of barriers weak to moderate for bacteria Streptococcus pyogenes and weak to strong resistance categories for Staphylococcus aureus bacteria.