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Chlorella vulgaris is a microalgae which is potent to be produced as beneficent healthcare product. Chlorella vulgaris has an antibacterial activity due to its fatty acid contents. The purpose of this research was to verify the antibacterial activity of Chlorella vulgaris against Propionibacterium acnes. Chlorella vulgaris is grown in Walne medium and cultivated in room temperature (±25-27°C), with photoperiod of 12:12 (dark:bright), light intensity of 10.000 lux and 24 hours aeration. Multilevel maceration was performed as the extraction method using n-hexane, chloroform and ethanol. The activity test was performed using Broth Microdilution in concentration of 65.536 µg/mL and 64 µg/mL was found to be the smallest concentration by dilution. The result shows the minimum inhibition concentration (MIC) is 8.192 µg/mL and minimum bactericidal concentration (MBC) is 16.384 µg/mL in extract of nhexane. Bioautography was performed to identify the antibacterial active compounds which is fatty acid of galactolipid. Antibacterial activity of Chlorella vulgaris against P. acnes is potential to be developed in emulgel dosage form. The ability of Chlorella vulgaris emulgel against P. acnes in concentration of 8% with inhibition zone of 12,63 mm. Based on the results, it is concluded that extract and emulgel of Chlorella vulgaris has antiacne activity against Propionibacterium acnes.
Keywords : Antiacnes, Propionibacterium acnes, Chlorella vulgaris, Emulgel