Phytochemical Screening and Antibacterial Activity of Ethanolic Extract of Syzygium samarangense Leaves

  • Nur Amalia Choironi Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman
  • Muhamad Salman Fareza Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman
Keywords: antibacterial, microdillution, Syzygium samarangense, MIC


The ethanolic extracts of S. samarangense leaves were examined for antibacterial activity in vitro using broth microdillution method. The extracts showed effective against Bacillus cereus, Salmonella enterica with MIC (minimum inhibition concentration) value 78 µg/ml and against multi strains of Escherichia coli, Enterobacter aerogenes, Kocuria rhizophila with MIC value 2500 µg/mL, 2500 µg/mL and 156 µg/mL. Phytochemical screening of S. samarangense was observed for the presence of tannins, alkaloids, flavonoids, and terpenoids.


Simirgiotis, M.J., Adachi, S., To, S., Yang, H., Reynertson, K.A., Basile, M.J., Gil, R.R., Weistein, I.B. and Kennelly, E.J., 2007, Cytotoxic chalcones and antioxidants from the fruits of S. samarangense (Wax Jambu), Food Chemistry, 107: 813-819.
[2] Lim, T.K. 2003. Edible Medicinal and Non-Edible Medicinal, Fruits. Springer Dordrecht Heidelberg London New York. 3: 778-786.
[3] Hanshella, C., Magno, R., Villasenor, I.M., Harada, N. and Monde, K., 2005, Antihyperglycaemic Flavonoids from Syzygium samarangense (Blume) Maerr and Perry, Phytotherapy Research, 19: 246-251.
[4] Peter, T., Padmavathi, D., Sajini, R.J., Sarala, A., 2011, Syzygium samarangense: A Review on Morphology, Phytochemistry and Pharmacological Aspects, Asian Journal of Biochemical and Pharmaceutical Research, 4 (1): 155-163.
[5] Srivastava, R., Arun, K., Shaw and Kulshreshtha, K., 1994, Triterpenoids and Chalcone from Syzygium samarangense, Phytochemistry, 38 (3): 687-689. 62: 1-5.
[6] Kuo, Y.C., Yang, L.M., Lin, L.C., 2004, Isolation and Immunomodulatory Effect of Flavonoids from Syzygium samarangense, Planta Medica, 70 (12): 1237-1239.
[7] Reddy, L.J. and Rose, B., 2011, Chemical Composition and Antibacterial Activity of The Volatile Oil from The Leave of Syzygium samarangense (Blum) Merr. & L.M. Perry, Asian Journal of Biochemical and Pharmaceutical Research, 3 (1): 263-269.
[8] Nair, A.G.R., Krishnan, S., Ravikrishna, C. and Madhusudanan, K.P., 1998, New and Rare Flavonol Glycosides from Leaves of Syzygium samarangense, Fitoterapia, 70: 148-151.
[9] Ragasa, C.Y., Franco, F.C., Raga, D.D. and Shen, C., 2014, Chemical Constituen of Syzygium samarangense, Deer Pharma Chemica, 6 (3): 256-260.
[10] Farnsworth, N.R., 1966, Biological and Phytochemical Screening of Plants, Journal of Pharmaceutical Sciences, 55 (3): 225-276.
[11] Zuhrotun A., Suganda AG., Sukandar EY., 2017, Toxicity of Selected Apocynaceae, Magnoliaceae and Simaroubaceae of Indonesian Plants Using Brine Shrimp Lethality Bioassay, Research Journal of Pharmaceutical, Biological and Chemical Sciences, 8: 10-15.
[12] Muthoharoh A., Zainab, 2015, Phytochemical Screening, Determination of Napthoquinone Content and Antifungal Activity of Insoluble Ethyl Acetate Fraction of Ethanolic Extract of Lawsonia inermis L. Leave Against Candida Albicans ATCC 10231, Pharmaciana, 5 (2): 199-208.
[13] Cockerill, F. R., Wikler, M. A., Alder, J., Dudley, M. N., Elipoulos, G. M. and Ferraro, M. J., 2012, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically: Approved Standard-Ninth Edition, Clinical and Laboratory Standars Institute, USA. 32 (2): 52-53.
[14] Klancnik, A., Piskernik, S., Jersek, B., Mozina, SS., 2010, Evaluation of Diffusion and Dilution Methods to Determine The Antibacterial Activity of Plant Extracts, Journal of Microbiological methods, 81 (2): 121-126.
[15] Eloff, J. N., 1998, A Sensitive and Quick Microplate Method to Determine the Minimal Inhibitory Concentration of Plant Extracts for Bacteria, Planta Medica, 64: 711-713.
[16] Abreu, A.C., McBain, A.J. and Simoes, M., 2012, Plants as Sources of Nes Antimicrobials and Resistance-modifying Agents, Natural Product Rep, 29: 1007-1021.
[17] Rios, J.L. and Recio, M.C., 2005, Medicinal Plants and Antimicrobial Activity, Journal of Ethnopharmacology, 100: 80-84.
[18] Ratnam, K.V. and Raju, R.R.V., 2008, In Vitro Antimicrobial Screening of The Fruit Extracts of Two Syzygium Species (Myrtaceae), Advances in Biological Research, 2 (1-2): 17-20.
[19] Cushnie, T.P.T., Lamb, A.J., 2005, Antimicrobial activity of Flavonoids, International Journal of Antimicrobial Agents, 26: 343-356.
[20] Pawar, M.M., Patil, S.D., Jadhav, A.P., Kadam, V.J., 2015, In vitro Antimicrobial Activity and Phytochemical Screening of Selected Indian Medicinal Plant, Indo American Journal of Pharmaceutical Research, 5 (1): 593-600.
[21] Oliveira, G.F., Furtado, N.A.J.C., Filho, A.A.S., Martins, C.H.G., Bastos, J.K., Cunha, W.R. and Silva, M.L.A., 2007, Antimicrobial activity of Syzygium cumini (Myrtaceae) Leaves Extract, Brazillian Journal of Microbiology, 38: 381-384.
[22] Silva, N.L., Zimmer, K.R., Macedo, A.J. and Trentin, D.S., 2016, Plant Natural Products Targeting Bacterial Virulences Factors, Chemical Reviews, 116: 9162-9236.
[23] Saleem, M., Nazir, M., Ali, M.S., Hussain, H., Lee, Y.S., Riaz, N., Jabbar, A., 2009, Natural Product Report,. 27: 238-254.
[24] Threlfall, E.J., Ward, L.R., Frost, J.A., Willshaw, 2000, The Emergence and Spread of Antibiotic Resistance in Food-borne Bacteria, International Journal of Food Microbiology, 768-789.
[25] Alzoreky, N.S. and Altaher, A.A., 2014, Antibacterial Activity of Spathe from Phoenix dactylifera Against Food-borne Pathogens, Industrial Crops and Products, 65: 241-246.
How to Cite
Choironi, N., & Fareza, M. (2018). Phytochemical Screening and Antibacterial Activity of Ethanolic Extract of Syzygium samarangense Leaves. Jurnal Kartika Kimia, 1(1), 1-4.
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